Anti– -enolase Antibodies in Patients with Inflammatory Bowel Disease

نویسندگان

  • Nathalie Vermeulen
  • Ingrid Arijs
  • Sofie Joossens
  • Severine Vermeire
  • Stefan Clerens
  • Karolien Van den Bergh
  • Georges Michiels
  • Lutgarde Arckens
  • Frans Schuit
  • Leentje Van Lommel
  • Paul Rutgeerts
  • Xavier Bossuyt
چکیده

METHODS: We used a classic proteomic approach with extracts from granulocytes and pANCA-positive ulcerative colitis (UC) sera to confirm -enolase as a target antigen. By means of Western blot analysis, we screened a cohort of 525 subjects for the presence of anti– -enolase antibodies. We performed GeneArray experiments on RNA extracted from colonic mucosal biopsies from 35 IBD and 6 control patients.

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تاریخ انتشار 2008